Reporter

Part:BBa_K362063:Design

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-25)


yaiA promoter - RBS - GFP - Term - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 351
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 668
    Illegal BamHI site found at 141
    Illegal BamHI site found at 657
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 351
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 351
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1360


Design Notes

This composite part contains yaiA promoter(BBa_K362004),RBS(BBa_K121013),GFP(BBa_K121013) and 2 terminators on low copy vector(BBa_K121013). yaiA promoter has sensitibity to oxydative stress.



Source

yaiA promoter from E.coli genome

References